Devil Hunter Yohko (魔物ハンター妖子 Mamono Hantā Yōko) is an anime series created by Madhouse and published in North America by ADV Films.

Devil Hunter Yohko is about a boy-crazy sixteen year-old girl named Yohko Mano (真野妖子 Mano Yōko) who banishes demons from the Earth. Yohko is played by Aya Hisakawa in the original Japanese dialogue. In the English dubbed version she is portrayed by Amanda Winn-Lee.

For centuries, the Mano family has been slaying demons. Yohko's grandmother, Madoka, is the one hundred-seventh Devil Hunter, and Yohko's mother, Sayoko, would have been the one hundred-eighth, but for a small hitch: A Devil Hunter must be a virgin to take on the power and responsibility. Sayoko became pregnant before Madoka could reveal the family's secrets, and so the job fell to Yohko, who had nearly been deflowered herself by her friend Osamu, who had been possessed by a demon thanks to the efforts of the school principal. But it wasn't until Yohko's friend Chigako Ogawa (Ogawa Chigako, also pronounced "Chikako") was endangered and Madoka was believed dead that Yohko took the ring that gave her the ability to transform into a Devil Hunter.